The leaves of Morus alba L. are an important herbal medicine in Asia. The systematic\nisolation of the metabolites of the leaves of Morus alba L. was achieved using a combination of\nliquid chromatography techniques. The structures were elucidated by spectroscopic data analysis\nand the absolute configuration was determined based on electronic circular dichroism (ECD)\nspectroscopic data and hydrolysis experiments. Their biological activity was evaluated using different\nbiological assays, such as the assessment of their capacity to inhibit the aldose reductase enzyme;\nthe determination of their cytotoxic activity and the evaluation of their neuroprotective effects against\nthe deprivation of serum or against the presence of nicouline. Chemical investigation of the leaves\nof Morus alba L. resulted in four new structures 1ââ?¬â??4 and a known molecule 5. Compounds 2 and\n5 inhibited aldose reductase with IC50 values of 4.33 Ã?¼M and 6.0 Ã?¼M compared with the potent\nAR inhibitor epalrestat (IC50 1.88 Ã?â?? 10âË?â??3 Ã?¼M). Pretreatment with compound 3 decreased PC12 cell\napoptosis subsequent serum deprivation condition and pretreatment with compound 5 decreased\nnicouline-induced PC12 cell apoptosis as compared with control cells (p < 0.001).
Loading....